Extracellular Vesicles by Winston Patrick Kuo & Shidong Jia

Author:Winston Patrick Kuo & Shidong Jia
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

Transmission electron microscopy (negative staining)

Cryo transmission electron microscopy

Advantages

• Simple sample preparation on grid

• Good signal-to-noise ratio

• Radiation resistant

• No artifacts due to fixation/dehydration

• Preservation of native conformation

• No stain is needed for visualization

Disadvantages

• Structural artifacts due to staining and dehydration

• Imaging under a non-native environment

• Technical demands in preparing, handling, and storing frozen samples prior to imaging

• Low signal-to-noise ratio due to ice thickness

• Freezing artifacts

• Radiation sensitivity

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